DescriptionThe GenScript Ni-Charged MagBeads are developed for quick and efficient small-scale purification of polyhistidine-tagged proteins under native or denaturing conditions.Perform cell lysis under native or denaturing conditions. Add the cell lysate containing your polyhistidine-tagged proteins to the Ni-Charged MagBeads and allow the proteins bind to the MagBeads. Then the isolated proteins can be eluted from the beads. Magnetic separation eliminates the need for multiple tubes, minimizes the loss of sample and removes several steps of the centrifugation process. The GenScript Ni-Charged MagBeads are average 40 µm in size, super paramagnetic beads with strong metal-chelating agent covalently bound to their surfaces. They are pre-charged with nickel and ready to use for quick and small-scale purification of polyhistidine-tagged proteins. The beads are supplied as 25% slurry in phosphate buffered saline (PBS), pH 7.4, containing 20% ethanol and 1 mM NiSO4. The Ni-Charged MagBeads have a binding capacity of 40 mg 6×His-tagged protein per 1 mL settled beads (e.g. 4 mL 25% slurry).產品概述Key FeaturesKey FeaturesQuick and convenient separation accomplished by magnetic forceHigh binding capacity with 40 mg of 6xHis-tagged protein/ml settled beadsCompatible with various reagents needed in the purification process (see table 1)Can be regenerated for multiple usesExtremely low nonspecific bindingTable 1 Reagents Compatible with Ni-charged Magnetic BeadsDenaturantsDetergentsReducing agentsSaltsOthers6 M Gu•HCl2% Triton X-10020 mM β-ME4 M MgCl250% glycerol8 M Urea2% Tween 201 mM DTT5 mM CaCl220% ethanol1% CHAPS2 M NaCl1 mM EDTA返回產品屬性Storage & StabilityThis product is stable until the expiration date stated on the COA, when stored unopened at 2–8°C. Do NOT freeze the product. Keep the MagBeads in liquid suspension during storage and all handling steps. Drying will cause loss of binding capacity and result in reduced performance. Resuspend the beads well before use. Be careful to avoid bacterial/fungal contamination.Description

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